【英文标准名称】:StandardPracticeforDetectionofMycoplasmaContaminationofBovineSerumbyLargeVolumeMethod
【原文标准名称】:用大容量试验法探测牛血清支原体污染物的标准实施规程
【标准号】:ASTME1536-2000(2006)
【标准状态】:现行
【国别】:
【发布日期】:2000
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E48.02
【标准类型】:(Practice)
【标准水平】:()
【中文主题词】:污染;医学科学;微生物学;试验
【英文主题词】:mycoplasma;serum
【摘要】:Mycoplasmasofbovineoriginareprevalentcontaminantsofcellcultures.Contaminationcanbedetectedbythelargevolumemethod.3,4Heatinactivatedserumneednotbetestedformycoplasmas.Heatingserumto56x00B0;Cfor30minwillkillmycoplasmas.Mycoplasmasmaybepresentinanyparticularlotofserumbutmaynotbedetectedbecauseofinadequatesamplesize;thus,negativetestresultsdonotprovideabsoluteassurancethatthetestserumisfreeofmycoplasmas.1.1Thispracticecoverstheproceduresusedfordetectionofmycoplasmacontaminationinserumbydirectmicrobiologicalculture.1.2Thispracticedoesnotcoverproceduresusedfordetectionofmycoplasmaincellcultures.1.3Thispracticedoesnotcoverindirectmethodsfordetectionofmycoplasmacontamination.1.4Thispracticedoesnotcovermethodsforidentificationofmycoplasmacultures.Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:C05
【国际标准分类号】:07_100_10
【页数】:2P.;A4
【正文语种】: